Media development for large scale Agrobacterium tumefaciens culture.
Identifieur interne : 000222 ( Main/Exploration ); précédent : 000221; suivant : 000223Media development for large scale Agrobacterium tumefaciens culture.
Auteurs : Ingrid K. Leth ; Karen A. McdonaldSource :
- Biotechnology progress [ 1520-6033 ] ; 2017.
Descripteurs français
- KwdFr :
- Acides aminés (métabolisme), Agrobacterium tumefaciens (génétique), Agrobacterium tumefaciens (métabolisme), Ammoniac (métabolisme), Biomasse (MeSH), Milieux de culture (composition chimique), Milieux de culture (métabolisme), Protéines recombinantes (analyse), Protéines recombinantes (génétique), Protéines recombinantes (métabolisme), Saccharose (métabolisme).
- MESH :
- analyse : Protéines recombinantes.
- composition chimique : Milieux de culture.
- génétique : Agrobacterium tumefaciens, Protéines recombinantes.
- métabolisme : Acides aminés, Agrobacterium tumefaciens, Ammoniac, Milieux de culture, Protéines recombinantes, Saccharose.
- Biomasse.
English descriptors
- KwdEn :
- Agrobacterium tumefaciens (genetics), Agrobacterium tumefaciens (metabolism), Amino Acids (metabolism), Ammonia (metabolism), Biomass (MeSH), Culture Media (chemistry), Culture Media (metabolism), Recombinant Proteins (analysis), Recombinant Proteins (genetics), Recombinant Proteins (metabolism), Sucrose (metabolism).
- MESH :
- chemical , analysis : Recombinant Proteins.
- chemical , chemistry : Culture Media.
- chemical , genetics : Recombinant Proteins.
- chemical , metabolism : Amino Acids, Ammonia, Culture Media, Recombinant Proteins, Sucrose.
- genetics : Agrobacterium tumefaciens.
- metabolism : Agrobacterium tumefaciens.
- Biomass.
Abstract
A chemically defined media was developed for growing Agrobacterium tumefaciens at large scale for commercial production of recombinant proteins by transient expression in plants. Design of experiments was used to identify major and secondary effects of ten media components: sucrose, ammonium sulfate ((NH4 )2 SO4 ), magnesium sulfate heptahydrate (MgSO4 *7H2 O), calcium chloride dihydrate (CaCl2 *2H2 O), iron (II) sulfate heptahydrate (FeSO4 *7H2 O), manganese (II) sulfate monohydrate (MnSO4 *H2 O), zinc sulfate heptahydrate (ZnSO4 *7H2 O), sodium chloride (NaCl), potassium chloride (KCl) and a sodium/potassium phosphate buffer (Na2 HPO4 /KH2 PO4 ). Calcium and zinc were found to have no detectable impact on biomass concentration or transient expression level, and concentrations of the other components that maximized final biomass concentration were determined. The maximum specific growth rate of Agrobacterium strain C58C1 pTFS40 in this media was 0.33 ± 0.01 h-1 and the final biomass concentration after 26 h of batch growth in shake flasks was 2.6 g dry cell weight/L. Transient expression levels of the reporter protein GUS following infiltration of a recombinant Agrobacterium strain C58C1 into N. benthamiana were comparable when the strain was grown in the defined media, Lysogeny Broth (LB) media, or yeast extract-peptone (YEP) media. In LB and YEP media, free amino acid concentration was measured at three points over the course of batch growth of Agrobacterium strain C58C1 pTFS40; results indicated that l-serine and l-asparagine were depleted from the media first, followed by l-alanine and l-glutamic acid. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1218-1225, 2017.
DOI: 10.1002/btpr.2504
PubMed: 28556626
Affiliations:
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Mcdonald</name><affiliation><nlm:affiliation>Dept. of Chemical Engineering, University of California at Davis, Davis, CA, 95616.</nlm:affiliation><wicri:noCountry code="subField">95616</wicri:noCountry></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2017">2017</date><idno type="RBID">pubmed:28556626</idno><idno type="pmid">28556626</idno><idno type="doi">10.1002/btpr.2504</idno><idno type="wicri:Area/Main/Corpus">000232</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000232</idno><idno type="wicri:Area/Main/Curation">000232</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000232</idno><idno type="wicri:Area/Main/Exploration">000232</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Media development for large scale Agrobacterium tumefaciens culture.</title><author><name sortKey="Leth, Ingrid K" sort="Leth, Ingrid K" uniqKey="Leth I" first="Ingrid K" last="Leth">Ingrid K. Leth</name><affiliation><nlm:affiliation>Dept. of Chemical Engineering, University of California at Davis, Davis, CA, 95616.</nlm:affiliation><wicri:noCountry code="subField">95616</wicri:noCountry></affiliation></author><author><name sortKey="Mcdonald, Karen A" sort="Mcdonald, Karen A" uniqKey="Mcdonald K" first="Karen A" last="Mcdonald">Karen A. Mcdonald</name><affiliation><nlm:affiliation>Dept. of Chemical Engineering, University of California at Davis, Davis, CA, 95616.</nlm:affiliation><wicri:noCountry code="subField">95616</wicri:noCountry></affiliation></author></analytic><series><title level="j">Biotechnology progress</title><idno type="eISSN">1520-6033</idno><imprint><date when="2017" type="published">2017</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium tumefaciens (genetics)</term><term>Agrobacterium tumefaciens (metabolism)</term><term>Amino Acids (metabolism)</term><term>Ammonia (metabolism)</term><term>Biomass (MeSH)</term><term>Culture Media (chemistry)</term><term>Culture Media (metabolism)</term><term>Recombinant Proteins (analysis)</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (metabolism)</term><term>Sucrose (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Acides aminés (métabolisme)</term><term>Agrobacterium tumefaciens (génétique)</term><term>Agrobacterium tumefaciens (métabolisme)</term><term>Ammoniac (métabolisme)</term><term>Biomasse (MeSH)</term><term>Milieux de culture (composition chimique)</term><term>Milieux de culture (métabolisme)</term><term>Protéines recombinantes (analyse)</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (métabolisme)</term><term>Saccharose (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Amino Acids</term><term>Ammonia</term><term>Culture Media</term><term>Recombinant Proteins</term><term>Sucrose</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Agrobacterium tumefaciens</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium tumefaciens</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Acides aminés</term><term>Agrobacterium tumefaciens</term><term>Ammoniac</term><term>Milieux de culture</term><term>Protéines recombinantes</term><term>Saccharose</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Biomass</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Biomasse</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A chemically defined media was developed for growing Agrobacterium tumefaciens at large scale for commercial production of recombinant proteins by transient expression in plants. Design of experiments was used to identify major and secondary effects of ten media components: sucrose, ammonium sulfate ((NH<sub>4</sub> )<sub>2</sub> SO<sub>4</sub> ), magnesium sulfate heptahydrate (MgSO<sub>4</sub> *7H<sub>2</sub> O), calcium chloride dihydrate (CaCl<sub>2</sub> *2H<sub>2</sub> O), iron (II) sulfate heptahydrate (FeSO<sub>4</sub> *7H<sub>2</sub> O), manganese (II) sulfate monohydrate (MnSO<sub>4</sub> *H<sub>2</sub> O), zinc sulfate heptahydrate (ZnSO<sub>4</sub> *7H<sub>2</sub> O), sodium chloride (NaCl), potassium chloride (KCl) and a sodium/potassium phosphate buffer (Na<sub>2</sub> HPO<sub>4</sub> /KH<sub>2</sub> PO<sub>4</sub> ). Calcium and zinc were found to have no detectable impact on biomass concentration or transient expression level, and concentrations of the other components that maximized final biomass concentration were determined. The maximum specific growth rate of Agrobacterium strain C58C1 pTFS40 in this media was 0.33 ± 0.01 h<sup>-1</sup> and the final biomass concentration after 26 h of batch growth in shake flasks was 2.6 g dry cell weight/L. Transient expression levels of the reporter protein GUS following infiltration of a recombinant Agrobacterium strain C58C1 into N. benthamiana were comparable when the strain was grown in the defined media, Lysogeny Broth (LB) media, or yeast extract-peptone (YEP) media. In LB and YEP media, free amino acid concentration was measured at three points over the course of batch growth of Agrobacterium strain C58C1 pTFS40; results indicated that l-serine and l-asparagine were depleted from the media first, followed by l-alanine and l-glutamic acid. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1218-1225, 2017.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">28556626</PMID><DateCompleted><Year>2018</Year><Month>06</Month><Day>15</Day></DateCompleted><DateRevised><Year>2018</Year><Month>06</Month><Day>15</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1520-6033</ISSN><JournalIssue CitedMedium="Internet"><Volume>33</Volume><Issue>5</Issue><PubDate><Year>2017</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Biotechnology progress</Title><ISOAbbreviation>Biotechnol Prog</ISOAbbreviation></Journal><ArticleTitle>Media development for large scale Agrobacterium tumefaciens culture.</ArticleTitle><Pagination><MedlinePgn>1218-1225</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1002/btpr.2504</ELocationID><Abstract><AbstractText>A chemically defined media was developed for growing Agrobacterium tumefaciens at large scale for commercial production of recombinant proteins by transient expression in plants. Design of experiments was used to identify major and secondary effects of ten media components: sucrose, ammonium sulfate ((NH<sub>4</sub> )<sub>2</sub> SO<sub>4</sub> ), magnesium sulfate heptahydrate (MgSO<sub>4</sub> *7H<sub>2</sub> O), calcium chloride dihydrate (CaCl<sub>2</sub> *2H<sub>2</sub> O), iron (II) sulfate heptahydrate (FeSO<sub>4</sub> *7H<sub>2</sub> O), manganese (II) sulfate monohydrate (MnSO<sub>4</sub> *H<sub>2</sub> O), zinc sulfate heptahydrate (ZnSO<sub>4</sub> *7H<sub>2</sub> O), sodium chloride (NaCl), potassium chloride (KCl) and a sodium/potassium phosphate buffer (Na<sub>2</sub> HPO<sub>4</sub> /KH<sub>2</sub> PO<sub>4</sub> ). Calcium and zinc were found to have no detectable impact on biomass concentration or transient expression level, and concentrations of the other components that maximized final biomass concentration were determined. The maximum specific growth rate of Agrobacterium strain C58C1 pTFS40 in this media was 0.33 ± 0.01 h<sup>-1</sup> and the final biomass concentration after 26 h of batch growth in shake flasks was 2.6 g dry cell weight/L. Transient expression levels of the reporter protein GUS following infiltration of a recombinant Agrobacterium strain C58C1 into N. benthamiana were comparable when the strain was grown in the defined media, Lysogeny Broth (LB) media, or yeast extract-peptone (YEP) media. In LB and YEP media, free amino acid concentration was measured at three points over the course of batch growth of Agrobacterium strain C58C1 pTFS40; results indicated that l-serine and l-asparagine were depleted from the media first, followed by l-alanine and l-glutamic acid. © 2017 American Institute of Chemical Engineers Biotechnol. Prog., 33:1218-1225, 2017.</AbstractText><CopyrightInformation>© 2017 American Institute of Chemical Engineers.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Leth</LastName><ForeName>Ingrid K</ForeName><Initials>IK</Initials><AffiliationInfo><Affiliation>Dept. of Chemical Engineering, University of California at Davis, Davis, CA, 95616.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>McDonald</LastName><ForeName>Karen A</ForeName><Initials>KA</Initials><Identifier Source="ORCID">http://orcid.org/0000-0002-5145-9968</Identifier><AffiliationInfo><Affiliation>Dept. of Chemical Engineering, University of California at Davis, Davis, CA, 95616.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2017</Year><Month>06</Month><Day>21</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Biotechnol Prog</MedlineTA><NlmUniqueID>8506292</NlmUniqueID><ISSNLinking>1520-6033</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000596">Amino Acids</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>57-50-1</RegistryNumber><NameOfSubstance UI="D013395">Sucrose</NameOfSubstance></Chemical><Chemical><RegistryNumber>7664-41-7</RegistryNumber><NameOfSubstance UI="D000641">Ammonia</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D016960" MajorTopicYN="N">Agrobacterium tumefaciens</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000596" MajorTopicYN="N">Amino Acids</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000641" MajorTopicYN="N">Ammonia</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018533" MajorTopicYN="N">Biomass</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013395" MajorTopicYN="N">Sucrose</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Agrobacterium tumefaciens</Keyword><Keyword MajorTopicYN="N">chemically defined medium</Keyword><Keyword MajorTopicYN="N">transient expression</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2016</Year><Month>11</Month><Day>29</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2017</Year><Month>05</Month><Day>03</Day></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2017</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2018</Year><Month>6</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2017</Year><Month>5</Month><Day>31</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">28556626</ArticleId><ArticleId IdType="doi">10.1002/btpr.2504</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list></list><tree><noCountry><name sortKey="Leth, Ingrid K" sort="Leth, Ingrid K" uniqKey="Leth I" first="Ingrid K" last="Leth">Ingrid K. Leth</name><name sortKey="Mcdonald, Karen A" sort="Mcdonald, Karen A" uniqKey="Mcdonald K" first="Karen A" last="Mcdonald">Karen A. Mcdonald</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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